Purification, stabilization and characte
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Shoji Ida; Eigo Mori; Yuhei Morita
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Article
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1974
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Springer-Verlag
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English
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Nitrite reductase (NiR) isolated from barley (Hordeum vulgare L.) roots was stabilized in a buffer solution containing a sulfhydryl-reducing reagent and glycerol. The enzyme was purified 340fold by ammonium sulfate fractionation and chromatography on DEAE-Sephadex A-50, Sephadex G-200 and DEAE-cellu