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Purification and characterisation of barley leaf nitrite reductase

โœ Scribed by Sin-Ming Ip; Judith Kerr; W.John Ingledew; John L. Wray


Book ID
119136578
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
842 KB
Volume
66
Category
Article
ISSN
0168-9452

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Purification, stabilization and characte
โœ Shoji Ida; Eigo Mori; Yuhei Morita ๐Ÿ“‚ Article ๐Ÿ“… 1974 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 712 KB

Nitrite reductase (NiR) isolated from barley (Hordeum vulgare L.) roots was stabilized in a buffer solution containing a sulfhydryl-reducing reagent and glycerol. The enzyme was purified 340fold by ammonium sulfate fractionation and chromatography on DEAE-Sephadex A-50, Sephadex G-200 and DEAE-cellu