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Pulsed optoacoustic spectroscopy of NADH

✍ Scribed by M.R. Fisher; N.S. Nogar; S.M. Schuster

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
450 KB
Volume
113
Category
Article
ISSN
0003-2697

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✦ Synopsis

Optoacoustic spectroscopy is a potentially powerful tool for the determination of trace and ultratrace components of biochemical interest. We derive here an abbreviated theory of pulsed optoacoustic spectroscopy, describe the experimental apparatus, and report on the performance in the determination of NADH. Results are consistent with theory over the concentration range 1 x 10e5 to 5 X 10-r M. A projected lower limit for detection is 1 x 10e8 M. Possible applications to the study of nucleotide interactions are discussed.

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