The use of pulsed potential waveforms to control antibody-antigen interactions on conducting polymer surfaces has been demonstrated. This enables detection of proteins at low levels and the use of antibody containing electrodes for multiple analyses.
Pulsed electrochemical detection of proteins using conducting polymer based sensors
β Scribed by W. Lu; H. Zhao; G.G. Wallace
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 443 KB
- Volume
- 315
- Category
- Article
- ISSN
- 0003-2670
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β¦ Synopsis
These studies reveal that sensitive responses for proteins can be generated at selected conducting polymer electrodes using flow-injection analysis (FIA) and pulsed electrochemical detection. The responses observed are dependent on the nature of the polymer, the nature of the electrolyte anion/cation as well as on the electrolyte concentration and pH. These factors can be used to modify selectivity. Detection limits in the order of nanomolar have been estimated.
π SIMILAR VOLUMES
The labelling on the y axis in Fig. 5 (p. 212) should be 0.5 PA, not 2.5 PA as shown.
In this article, we have demonstrated the feasibility of using dye binding interactions for the amperometric detection of proteins at conducting polymer coated electrodes with flow injection analysis. Incorporation of appropriate dyes into the conducting polymer during synthesis enables sensitive an