Pulsatilla decoction and its active ingredients inhibit secretion of NO, ET-1, TNF-α, and IL-1α in LPS-induced rat intestinal microvascular endothelial cells
✍ Scribed by Yiyi Hu; Xi Chen; Huiqin Duan; Yuanliang Hu; Xiang Mu
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 82 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0263-6484
- DOI
- 10.1002/cbf.1570
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✦ Synopsis
Abstract
To investigate the pharmacological mechanism of the traditional Chinese medicine, Pulsatilla decoction (PD), the levels of nitric oxide (NO), endothelin‐1 (ET‐1), tumor necrosis factor‐α (TNF‐α), and interleukin‐1__α__ (IL‐1__α__) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs) were determined after treatment with PD and its seven active ingredients, namely anemoside B~4~, anemonin, berberine, jatrorrhizine, palmatine, aesculin, and esculetin. RIMECs were challenged with lipopolysaccharide (LPS) at 1 µg ml^−1^ for 3 h and then treated with PD at 1, 5, and 10 mg ml^−1^ and its seven ingredients at 1, 5, and 10 µg ml^−1^ for 21 h, respectively. The results revealed that PD, anemonin, berberine, and esculetin inhibited the production of NO; PD, anemonin, and esculetin inhibited the secretion of ET‐1; PD, anemoside B~4~, berberine, jatrorrhizine, and aesculin downregulated TNF‐α expression; PD, anemoside B~4~, berberine, and palmatine decreased the content of IL‐1__α__. It showed that PD and its active ingredients could significantly inhibit the secretion of NO, ET‐1, TNF‐α, and IL‐1__α__ in LPS‐induced RIMECs and suggested they would reduce inflammatory response via these cytokines. Copyright © 2009 John Wiley & Sons, Ltd.