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pUBEX/pUBSEX: a versatile expression vector system for production of fusion and nonfusion proteins in Escherichia coli

โœ Scribed by Banting, George; Luzio, J.Paul; Braghetta, Paolo; Brake, Brigitte; Stanley, Keith K.

Book ID
123326849
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
832 KB
Volume
107
Category
Article
ISSN
0378-1119

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Covalently immobilized biotin was used as a biospecific adsorbant to investigate the application of streptavidin as an affinity domain for simultaneous purification and immobilization of recombinant proteins. A streptavidinp-galactosidase fusion protein was constructed and tested as a model system.