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pUB307 mobilizes resistance plasmids from Escherichia coli into Neisseria gonorrhoeae

✍ Scribed by Piffaretti, Jean-Claude ;Arini, Achille ;Frey, Joachim

Book ID: 104727436
Publisher: Springer
Year: 1988
Tongue: English
Weight: 413 KB
Volume: 212
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00334687

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✦ Synopsis

The plasmid pUB307, a derivative of RP1, is a conjugative, broad-host-range plasmid. We have shown that this element mobilizes gonococcal resistance plasmids from Escherichia coli to Neisseria gonorrhoeae, thus providing evidence that extrachromosomal elements can efficiently enter gonococci by conjugation. Furthermore, pUB307 can also be used as a helper element to mobilize the cloning vector pLES2 into N. gonorrhoeae. This finding significantly increases the usefulness of pLES2 as a shuttle vector between E. coli and gonococcus.

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