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Pseudoisokinetic analytical ultracentrifugation: Monitoring of bands at a fixed region in the analytical ultracentrifuge

✍ Scribed by Sidney Pestka; Daniel Luk

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 550 KB
Volume: 89
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90739-x

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✦ Synopsis

With the use of a Model E analytical ultracentrifuge equipped with a photoelectric scanner, a technique for obtaining isokinetic patterns of moving zones has been developed by monitoring a fixed area of the analytical cell as a function of time. Small amounts of material spread over a very wide range of sedimentation coefficients can be used in a single experiment. Since the resultant tracing is effectively isokinetic, sedimentation coefficients can be calculated by simple ratio if the S value of any component is known.

EXPERIMENTAL PROCEDURE Materials, chemicals, and cell extracts. Sucrose solutions were prepared from RNase-free sucrose. Ribosomes, ribosomal subunits, and poly-

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