Pseudocollagenase: A protease from Clostridium histolyticum
β Scribed by William M. Mitchell
- Book ID
- 115731691
- Publisher
- Elsevier Science
- Year
- 1968
- Weight
- 370 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0005-2744
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π SIMILAR VOLUMES
Clostripain (EC 3.4.22.8) is a heterodimeric cysteine endopeptidase with strict specificity for Arg-Xaa peptidyl bonds. It is secreted by Clostridium histolyticum strains. For the first time we present evidence that both polypeptide chains of native clostripain are encoded by a single gene. DNA sequ
A continuously recording spectrophotometric assay has been developed for Clostridium histolyticum collagenase based on the hydrolysis of 2-furanacryloyl-L-leucylglycyl-L-prolyl-L-alanine (FALGPA). The hydrolysis of this peptide by collagenase obeys Michaelis-Menten kinetics with V = 1.8 X IO5 pkatal