Provision of the immunoglobulin heavy chain enhancer downstream of a test gene is sufficient to confer lymphoid-specific expression in transgenic mice
✍ Scribed by Wolf Reik; Gareth Williams; Sheila Barton; Mike Norris; Michael Neuberger; M. Azim Surani
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 566 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
Immunoglobulin heavy chain kb: Kilo-base pair SV40: Simian virus 40 2 Materials and methods 2.1 Plasmid construction A derivative of plasmid pAlOCAT2 that contains a Bgl I1 linker in the unique SphI site [19] was kindly provided by C. Gorman and P. Gruss. The Bgl 11-Bam HI chloramphenicol acetyl transferase (CAT)-containing fragment of this plasmid was cloned into the unique Bain HI site of a pUC12derivative that includes 1 kilo-base pair (kb) Xba I fragment that spans the mouse IgH enhancer. The entire CAT-IgH transcription unit (see Fig. 1) could be excised from this plasmid by cleavage at the Sac I and Sal I sites in the polylinker.