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Prototypical Test Substances for a Reversed Phase in a Retention Parameter Model

✍ Scribed by Rohrschneider, Lutz

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 62 KB
Volume: 22
Category: Article
ISSN: 0935-6304
DOI: 10.1002/(sici)1521-4168(19990801)22:8<454::aid-jhrc454>3.0.co;2-#

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✦ Synopsis

A set of four prototypical substances (naphthalene, acetanilide, phenol, and benzonitrile) is selected from 26 different analytes used by Seibert and Poole for characterization of the retention behavior of 20 liquid chromatographic systems of a C18 reversed phase with methanol/water and acetonitrile/water as mobile phases. Retention data of 22 different analytes can be calculated from the retention data of the set of prototypical substances with a mean standard deviation of 5.4%. Two phase system maps similar to that suggested by Poole et al. are provided for an octadecylsiloxane-bonded silica sorbent with methanol and acetonitrile as cosolvents with water.

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