Proton-observed carbon-edited NMR spectroscopy in strongly coupled second-order spin systems
✍ Scribed by Pierre-Gilles Henry; Malgorzata Marjanska; Jamie D. Walls; Julien Valette; Rolf Gruetter; Kâmil Uǧurbil
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 295 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
Proton‐observed carbon‐edited (POCE) NMR spectroscopy is commonly used to measure ^13^C labeling with higher sensitivity compared to direct ^13^C NMR spectroscopy, at the expense of spectral resolution. For weakly coupled first‐order spin systems, the multiplet signal at a specific proton chemical shift in POCE spectra directly reflects ^13^C enrichment of the carbon attached to this proton. The present study demonstrates that this is not necessarily the case for strongly coupled second‐order spin systems. In such cases NMR signals can be detected in the POCE spectra even at chemical shifts corresponding to protons bound to ^12^C. This effect is demonstrated theoretically with density matrix calculations and simulations, and experimentally with measured POCE spectra of [3‐^13^C]glutamate. Magn Reson Med, 2006. © 2006 Wiley‐Liss, Inc.