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Proton NMR studies of the histidine residues and of bound ATP on rabbit muscle pyruvate kinase

✍ Scribed by Shunsuke Meshitsuka; Gary M. Smith; Albert S. Mildvan


Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
274 KB
Volume
20
Category
Article
ISSN
0020-7608

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Fully deuterated yeast phosphoglycerate kinase ([2H]PGK) was prepared biosynthetically with only histidine side chains of normal (1H) isotopic composition. The 1H NMR spectrum of this enzyme ([1H]His[2H]PGK) showed that the histidine side chains are clearly visible as sharp signals. Thus detailed st

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