PROTON, Mg2+ AND PROTEIN AS COMPETING LIGANDS FOR THE FLUORESCENT PROBE, MAG-INDO-1: A FIRST STEP TO THE QUANTIFICATION OF INTRACELLULAR Mg2+ CONCENTRATION

Mag-indo1 Affinity for Ca2+, Compartment

Mag-indo1 Affinity for Ca2+, Compartmentalization and Binding to Proteins: The Challenge of Measuring Mg2+ Concentrations in Living Cells

✍ Judith Pesco; Jean-Marie Salmon; Jean Vigo; Pierre Viallet 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 157 KB

A physicochemical study of the Mag-indo1 binding to Ca 2؉ in solution showed that: (i) the characteristic fluorescence spectra of Ca 2؉ -bound and Mg 2؉ -bound Mag-indo1 are identical; (ii) two successive equilibria occur for increasing Ca 2؉ concentrations; and (iii) the value of the dissociation c