Proton magnetic resonance study of complex formation between N6-methyladenosine and polyuridylic acid

✍ Scribed by Benedict W. Bangerter; Zivile Modestas

• Publisher: Wiley (John Wiley & Sons)
• Year: 1974
• Tongue: English
• Weight: 467 KB
• Volume: 13
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1974.360130309

✦ Synopsis

Abstract

The interaction between N~6~‐methyladenosine and polyuridylic acid in D~2~O solution at neutral pD has been studied as a function of temperature and N~6~‐methyladenosine concentration by proton magnetic resonance spectroscopy. A rigid double‐stranded 1:1 complex is formed below ∼10°C, involving hydrogen‐bonded N~6~‐methyladenine:uracil base‐pairing and stacking of the adenine bases. This complex is less stable than the 1:2 complex formed between adenosine and polyU, and involves a more rapid exchange of the monomer between free and polymer‐bound environments.