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Proton and metal ion interactions with DNA and DNA-dye complexes: Stability and effects from cation binding

✍ Scribed by V.G. Bregadze; I.G. Khutsishvili; J.G. Chkhaberidze


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
61 KB
Volume
59
Category
Article
ISSN
0162-0134

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## Abstract The binding of Mg^2+^ and Mn^2+^ by DNA by a divalent cation specific electrode and by ultracentrifugation. Both techniques give similar results for the stoichiometry of the reaction. An oscillating densiemete allowed us to detect small changes of volume accompanying the binding. The re

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## Abstract DNA with Mn^2+^ as the only counterion has been prepared, and the extent of the Mn^2+^ binding was determined under a variety of conditions through measurements of the proton relaxation enhancement of water. The total extent of Mn^2+^ binding per DNA phosphate is found to be 0.43 Β± 0.04

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## Abstract It has been inferred from previous studies that Mn^2+^ ions bind preferentially to GΒ·C base pairs in DNA, and it has even been suggested that this preference for GΒ·C pairs might be responsible for some of the Mn^2+^ specific effects observed in various biochemical reactions. In this pap