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Proton: A Major Factor for the Racemization and the Dehydration at the Cyclization/Cleavage Stage in the Edman Sequencing Method

โœ Scribed by Matsunaga, Hirokazu; Santa, Tomofumi; Iida, Takayuki; Fukushima, Takeshi; Homma, Hiroshi; Imai, Kazuhiro


Book ID
121408580
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
281 KB
Volume
68
Category
Article
ISSN
0003-2700

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Boron trifluoride-etherate (BF,), one of the Lewis acids, was found to be an efficient acid in the Edman sequencing method, affording the retention of the N-terminal amino acid configuration at the cyclization/ cleavage reaction from peptides. Examples for the sequencing of o-ku-Gly and L-Pro-L-ku a