Protocols for Gene Analysis Volume 31 || Use of Silver Staining to Detect Nucleic Acids

✍ Scribed by Harwood, Adrian J.

• Book ID: 121361448
• Publisher: Humana Press
• Year: 1994
• Tongue: English
• Weight: 772 KB
• Edition: 1994
• Category: Article
• ISBN-13: 9780896032583
• DOI: 10.1385/0-89603-258-2:197

✦ Synopsis

Basic Recombinant Dna Techniques -- Transformation Of Bacteria By Electroporation -- Direct Cloning Of [lambda]g11 Cdna Inserts Into A Plasmid Vector -- Pcr Cloning Using T-vectors -- Thermal Cycle Dideoxy Dna Sequencing -- In Vitro Mutagenesis -- Ordered Deletions Using Exonuclease Iii -- Site-directed Mutagenesis Using A Double-stranded Dna Template -- Site-directed Mutagenesis Using A Uracil-containing Phagemid Template -- Construction Of Linker-scanning Mutations By Oligonucleotide Ligation -- Construction Of Linker Scanning Mutations Using The Polymerase Chain Reaction -- Localized Random Polymerase Chain Reaction Mutagenesis -- Genomic Structure -- Simultaneous Isolation Of Rna And Dna From Tissues And Cultured Cells. (cont) Physical Mapping Of The Human Genome By Pulsed Field Gel Electrophoresis -- Field Inversion Gel Electrophoresis -- Enhanced Chemiluminescent Detection Of Horseradish Peroxidase Labeled Probes -- Nonradioactive Oligonucleotide Probe Labeling -- Analysis Of Dna Restriction Enzyme Digests By Two-dimensional Electrophoresis In Agarose Gels -- Inverse Polymerase Chain Reaction -- Sequence Variations -- Use Of Silver Staining To Detect Nucleic Acids -- Nonradioactive Method For The Detection Of Single-strand Conformational Polymorphisms (sscp) -- Temperature Gradient Gel Electrophoresis (tgge) For The Detection Of Polymorphic Dna And Rna -- Tgge In Quantitative Pcr Of Dna And Rna -- Pgk-pcr Clonality Assay (ppca). (cont) Direct Sequencing Of Pcr Products -- Gene Expression -- Use Of Riboprobes For The Analysis Of Gene Expression -- Quantification Of Absolute Amounts Of Cellular Messenger Rna By Rna-excess Solution Hybridization -- Measurements Of Rate Of Transcription In Isolated Nuclei By Nuclear Run-off Assay -- Rna Polymerase Ii In Vitro Transcription System -- S1 Mapping Using Single-stranded Dna Probes -- Single Primer-mediated Polymerase Chain Reaction -- Protein-dna Interactions -- In Vivo Dna Footprinting By Linear Amplification -- Dna Photofootprinting With Rh(phi)₂bpy³⁺ -- Gel Retardation Assay -- Southwestern Assay. (cont) Cloning Dna Binding Proteins From Cdna Expression Libraries Using Oligonucleotide Binding Site Probes -- Protein Function -- 6xhis-ni-nta Chromatography As A Superior Technique In Recombinant Protein Expression/purification -- Production Of ³⁵s-labeled Proteins In E. Coli And Their Use As Molecular Probes -- Preparation And Ligand Screening Of A [lambda]g11 Lysogen Library. Edited By Adrian J. Harwood. Includes Bibliographical References And Index.