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Protocol for Somatic Embryogenesis in Woody Plants

✍ Scribed by Ronald J. Newton, Wei Tang, S. Mohan Jain (auth.), S. Mohan Jain, Pramod K. Gupta (eds.)


Publisher
Springer Netherlands
Year
2005
Tongue
English
Leaves
579
Series
Forestry Sciences 77
Edition
1
Category
Library

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✦ Synopsis


Earlier, we edited a well received series on "Somatic embryogenesis in woody trees", volumes 1 to 6. These volumes provided readers extensive reviews on somatic embryogenesis of important angiosperm and gymnosperm tree species; an excellent source of information for newcomers or those already engaged in research. However, these book volumes did not cover stepwise "detailed protocols" for inducing somatic embryogenesis. This book contains 46 chapters, divided into 4 sections: A) 12 chapters on conifers, B) 14 chapters on fruits, C) 14 chapters on angiosperms, and D) 6 chapters on histology, bioencapsulation, protoplasts, cryopreservation, double staining and thin cell layer sectioning. The book provides stepwise protocols for somatic embryogenesis of a range of selected woody plants in order to assist researchers to initiate somatic embryogenic cultures without too much alterations in protocols. Each chapter provides information on initiation and maintenance of embryogenic cultures; somatic embryo development, maturation, and germination; acclimitization and field transfer of somatic seedlings. Some chapteres include applications of somatic embryogenesis cultures, e.g. protoplasts, encapsulation, cryopreservation, genetic transformation, genetic fidelity with molecular markers, and bioreactor.

✦ Table of Contents


Slash Pine ( Pinus elliottii Engelm.)....Pages 1-10
Somatic Embryogenesis and Genetic Transformation in Pinus radiata ....Pages 11-24
Douglas - Fir ( Pseudotsuga menziesii )....Pages 25-34
Omorika Spruce ( Picea omorika )....Pages 35-45
Somatic Embryogenesis in Picea glauca ....Pages 47-58
Protocol of Somatic Embryogenesis: Black Spruce ( Picea mariana (Mill.) B.S.P.)....Pages 59-68
Sitka Spruce ( Picea sitchensis )....Pages 69-80
Protocol of Somatic Embryogenesis of Pinus nigra Arn.....Pages 81-93
Loblolly Pine ( Pinus taeda )....Pages 95-106
Somatic Embryogenesis in Maritime Pine ( Pinus pinaster Ait.)....Pages 107-119
Somatic Embryogenesis in Pinus patula ....Pages 121-139
Somatic Embryogenesis in Norway Spruce....Pages 141-155
Cashew ( Anacardium occidentale L.)....Pages 157-165
Somatic Embryogenesis Protocol: Coffee ( Coffea arabica L. and C. canephora P.)....Pages 167-179
Protocols for Somatic Embryogenesis and Plantlet Formation from Three Explants in Tea (C amellia sinensis (l.) o. kuntze )....Pages 181-190
Protocol of Somatic Embryogenesis from Citrus spp. Anther Culture....Pages 191-207
Integrated System for Propagation of Theobroma cacao L. ....Pages 209-227
Mango ( Mangifera indica L.)....Pages 229-246
Somatic Embryogenesis Injackfruit ( Artocarpus heterophyllus Lam.)....Pages 247-256
Somatic Embryogenesis in Indian Olive ( Elaeocarpus robustus L)....Pages 257-266
Rescue of Endangered Palms by in vitro Methods: The Case of β€˜Bottle Palm’....Pages 267-274
Somatic Embryogenesis in American Grapes ( Vitis x labruscana L.H. Bailey)....Pages 275-287
Pistachio (Pistacia vera L.)....Pages 289-300
Grape ( Vitis vinifera L.) ....Pages 301-308
Date Palm Phoenix dactylifera L.....Pages 309-319
Somatic Embryogenesis Protocol: Citrus ....Pages 321-343
Olive ( Olea europaea L.)....Pages 345-360
Protocol of Somatic Embryogenesis: Dalbergia sissoo Roxb. (Sissoo)....Pages 361-368
Protocol of Somatic Embryogenesis: Pedunculate Oak ( Quercus robur L.) and Sessile Oak ( Quercus petraea /Matt./ Liebl.)....Pages 369-378
Protocol of Somatic Embryogenesis: Tamarillo ( Cyphomandra betacea (Cav.) Sendtn.)....Pages 379-389
Protocol of Somatic Embryogenesis: European Chestnut ( Castanea sativa Mill.)....Pages 391-399
Protocol of Somatic Embryogenesis in Acacia arabica (Lamk.) Willd.....Pages 401-411
Protocol for Hazelnut Somatic Embryogenesis....Pages 413-426
Protocol of Somatic Embryogenesis: Ocotea catharinensis Mez. (Lauraceae)....Pages 427-443
Cork Oak, Quercus suber L.....Pages 445-457
Sawara Cypress Chamaecyparis pisifera Sieb. et Zucc.....Pages 459-468
Protocol of Somatic Embryogenesis: Holm Oak ( Quercus ilex L.)....Pages 469-482
Protocols for Somatic Embryogenesis of Hybrid Firs....Pages 483-496
Somatic Embryogenesis in Sandalwood....Pages 497-504
Echinacea purpurea L.: Somatic Embryogenesis from Leaf Explant....Pages 505-515
Histological Techniques....Pages 517-537
Bioencapsulation of Somatic Embryos in Woody Plants....Pages 539-552
Protoplast Isolation and Culture of Woody Plants....Pages 553-566
Cryopreservation of Embryonal Cells....Pages 567-572
Double Staining Technology for Distinguishing Embryogenic Cultures....Pages 573-575
Thin Cell Layer Sectioning for Inducing Somatic Embryogenesis in Woody Plants....Pages 577-585

✦ Subjects


Life Sciences, general; Tree Biology; Wood Science & Technology; Plant Anatomy/Development


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