Proteomics in Practice: A Guide to Successful Experimental Design, Second Edition

✍ Scribed by Dr. Reiner Westermeier, Dr. Tom Naven, Hans?Rudolf Hopker(auth.)

• Publisher: Wiley-Blackwell
• Year: 2008
• Tongue: English
• Leaves: 492
• Category: Library

✦ Synopsis

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.Content:
Chapter 1 History (pages 1–8):
Chapter 2 Critical Points (pages 8–12):
Chapter 3 Proteomics Strategies (pages 12–13):
Chapter 4 Concept of Experimental Planning (pages 14–15):
Chapter 1 Electrophoretic Techniques (pages 19–149):
Chapter 2 Liquid Chromatography Techniques (pages 151–213):
Chapter 3 Mass Spectrometry (pages 215–272):
Chapter 4 Functional Proteomics: Studies of Protein–Protein Interactions (pages 273–277):
Chapter none Equipment, Consumables, Reagents (pages 279–410):
Chapter 1 Two?Dimensional Electrophoresis (pages 411–428):
Chapter 2 Mass Spectrometry (pages 429–431):