Proteomics and peptidomics in neuroscience. Experience of capabilities and limitations in a neurochemical laboratory

✍ Scribed by Linda Paulson; Rita Persson; Gösta Karlsson; Jerzy Silberring; Anna Bierczynska-Krzysik; Rolf Ekman; Ann Westman-Brinkmalm

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 276 KB
• Volume: 40
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.740

✦ Synopsis

Abstract

The increasing use of proteomics has created a basis for new strategies to develop methodologies for rapid identification of protein patterns in living organisms. It has also become evident that proteomics has other potential applications than protein and peptide identification, e.g. protein characterization, with the aim of revealing their structure, function(s) and interactions of proteins. In comparative proteomics studies, the protein expression of a certain biological system is compared with another system or the same system under perturbed conditions. Global identification of proteins in neuroscience is extremely complex, owing to the limited availability of biological material and very low concentrations of the molecules. Moreover, in addition to proteins, there are number of peptides that must also be considered in global studies on the central nervous system. In this overview, we focus on and discuss problems related to the different sources of biological material and sample handling, which are part of all preparatory and analytical steps. Straightforward protocols are desirable to avoid excessive purification steps, since loss of material at each step is inevitable. We would like to merge the two worlds of proteomics/peptidomics and neuroscience, and finally we consider different practical and technical aspects, illustrated with examples from our laboratory. Copyright © 2005 John Wiley & Sons, Ltd.