✦ LIBER ✦

Proteomic profiling of distinct clonal populations of bone marrow mesenchymal stem cells

✍ Scribed by Shobha Mareddy; James Broadbent; Ross Crawford; Yin Xiao

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 281 KB
Volume: 106
Category: Article
ISSN: 0730-2312
DOI: 10.1002/jcb.22088

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Mesenchymal stem cells (MSCs) have attracted immense research interest in the field of regenerative medicine due to their ability to be cultured for successive passages and multi‐lineage differentiation. The molecular mechanisms governing MSC self‐renewal and differentiation remain largely unknown. The development of sophisticated techniques, in particular clinical proteomics, has enabled researchers in various fields to identify and characterize cell specific biomarkers for therapeutic purposes. This study seeks to understand the cellular and sub‐cellular processes responsible for the existence of stem cell populations in bone marrow samples by revealing the whole cell proteome of the clonal cultures of bone marrow‐derived MSCs (BMSCs). Protein profiling of the MSC clonal populations was conducted by Two‐Dimensional Liquid Chromatography/Matrix‐Assisted Laser Desorption/Ionisation (MALDI) Mass Spectrometry (MS). A total of 83 proteins were identified with high confidence of which 11 showed differential expression between subpopulations, which included cytoskeletal and structural proteins, calcium binding proteins, cytokinetic proteins, and members of the intermediate filament family. This study generated a proteome reference map of BMSCs from the clonal populations, which will be valuable to better understand the underlying mechanism of BMSC self‐renewal and differentiation. J. Cell. Biochem. 106: 776–786, 2009. © 2009 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Age-related gene expression profiles of

✍ Joseph G. Hacia; C. Chang I. Lee; Daniel F. Jimenez; Mazen W. Karaman; Vincent V 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 270 KB 👁 2 views

## Abstract The objective of this study was to elucidate age‐related differences in gene expression profiles of rhesus monkey bone marrow‐derived mesenchymal stem cells (rhMSC) obtained from fetal, infant, and adult donors relevant to their growth and other properties. Although a high degree of sim

Distinct osteoblastic differentiation po

Distinct osteoblastic differentiation potential of murine fetal liver and bone marrow stroma-derived mesenchymal stem cells

✍ Olivia Fromigué; Zahia Hamidouche; Sébastien Chateauvieux; Pierre Charbord; Pier 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 232 KB 👁 2 views

## Abstract Bone marrow‐derived mesenchymal stem cells (MSC) are able to differentiate into osteoblasts under appropriate induction. Although MSC‐derived osteoblasts are part of the hematopoietic niche, the nature of the stromal component in fetal liver remains elusive. Here, we determined the in v

Characterisation and developmental poten

Characterisation and developmental potential of ovine bone marrow derived mesenchymal stem cells

✍ Rosa C. McCarty; Stan Gronthos; Andrew C. Zannettino; Bruce K. Foster; Cory J. X 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 446 KB 👁 2 views

## Abstract Since discovery, significant interest has been generated in the potential application of mesenchymal stem cells or multipotential stromal cells (MSC) for tissue regeneration and repair, due to their proliferative and multipotential capabilities. Although the sheep is often used as a lar

Survival of bone marrow-derived mesenchy

Survival of bone marrow-derived mesenchymal stem cells in a xenotransplantation model

✍ Yan Wang; Xi Chen; Marilyn Ann Armstrong; Gang Li 📂 Article 📅 2007 🏛 Elsevier Science 🌐 English ⚖ 406 KB 👁 1 views

## Abstract Mesenchymal stem cells (MSCs) are immunoprivileged and the allogeneic MSCs implantation has been used to facilitate tissue repairs such as bone and cartilage defect. The present study aimed to investigate the feasibility of xenogeneic MSCs implantation. Green fluorescent protein (GFP) t

Expansion of mesenchymal stem cells isol

Expansion of mesenchymal stem cells isolated from pediatric and adult donor bone marrow

✍ Katia Mareschi; Ivana Ferrero; Deborah Rustichelli; Simona Aschero; Loretta Gamm 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 146 KB 👁 1 views

## Abstract The enormous plasticity of mesenchymal stem cells (MSCs) suggests an improvement of a standard protocol of isolation and ex vivo expansion for experimental and clinical use. We isolated and expanded MSCs from bone marrow (BM) of pediatric and young adult donors, to analyze the growth ki

Biologic properties of mesenchymal stem

Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue

✍ Reza Izadpanah; Cynthia Trygg; Bindiya Patel; Christopher Kriedt; Jason Dufour; 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 377 KB 👁 2 views

## Abstract The biologic characteristics of mesenchymal stem cells (MSCs) isolated from two distinct tissues, bone marrow and adipose tissue were evaluated in these studies. MSCs derived from human and non‐human primate (rhesus monkey) tissue sources were compared. The data indicate that MSCs isola