Proteomic analysis of the mouse brain following protein enrichment by preparative electrophoresis
β Scribed by Elena Xixi; Ploumisti Dimitraki; Kostantinos Vougas; Sofia Kossida; Gert Lubec; Michael Fountoulakis
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 373 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0173-0835
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Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry is a powerful combination for the separation of complex protein mixtures in biological samples and the subsequent identification of individual polypeptides. We have used this approach to construct a database of proteins of the
## Abstract This report describes a method for enrichment and separation of acidic and basic proteins using the centrifugal ultrafiltration followed by nanoparticleβfilled capillary electrophoresis. To improve stacking and separation efficiencies of proteins, the separation buffer containing 1.6% p