Proteomic analysis of selenium embryotoxicity in cultured postimplantation rat embryos
β Scribed by Makoto Usami; Katsuyoshi Mitsunaga; Ken Nakazawa; Osamu Doi
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 674 KB
- Volume
- 83
- Category
- Article
- ISSN
- 1542-9733
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Effects of depletion of reduced glutathione (GSH) on selenium (Se) embryotoxicity in cultured rat embryos were examined. Rat embryos at day 9.5 of gestation were cultured for 48 h in the presence of Se as either sodium selenite at 10 and 20 Β΅M or sodium selenate at 30 and 100 Β΅M. Embryonic GSH was d
## Abstract The growth factor for postimplantation rat embryos was investigated on the basis of the serum speciesβspecificity in supporting embryonic development in culture. We used rabbit serum as a basal medium for the culture of headβfold stage rat embryos, and examined the effects of various fr