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πŸ“

Proteome Research: Mass Spectrometry

✍ Scribed by Dr. Peter James (auth.)

Publisher
Springer-Verlag Berlin Heidelberg
Year
2001
Tongue
English
Leaves
286
Series
Principles and Practice
Edition
1
Category
Library
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✦ Synopsis

Recent advances in large-scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Two-dimensional gel electrophoresis can be used to create cellular protein maps which give a quantitative and qualitative picture of the proteome. Mass spectrometry is the method of choice for the rapid large-scale idenfification of these proteomes and their modifications. An understanding of these methods is critical for scientists in the "Post-Genome" era.

✦ Table of Contents

Front Matter....Pages I-XXI
Mass Spectrometry and the Proteome....Pages 1-9
Basics of Triple-Stage Quadrupole/Ion-Trap Mass Spectrometry: Precursor, Product and Neutral-Loss Scanning. Electrospray Ionisation and Nanospray Ionisation....Pages 11-31
The Basics of Matrix-Assisted Laser Desorption, Ionisation Time-of-Flight Mass Spectrometry and Post-Source Decay Analysis....Pages 33-53
Data-Controlled Micro-Scale Liquid Chromatography β€” Tandem Mass Spectrometry of Peptides and Proteins: Strategies for Improved Sensitivity, Efficiency and Effectiveness....Pages 55-74
Solid-Phase Extraction-Capillary Zone Electrophoresis-Mass Spectrometry Analysis of Low-Abundance Proteins....Pages 75-101
Protein Identification by Peptide-Mass Fingerprinting....Pages 103-123
Protein Identification by SEQUEST....Pages 125-142
Interpreting Peptide Tandem Mass-Spectrometry Fragmentation Spectra....Pages 143-166
Automated Interpretation of Peptide Tandem Mass Spectra and Homology Searching....Pages 167-185
Specific Detection and Analysis of Phosphorylated Peptides by Mass Spectrometry....Pages 187-206
Glycoproteomics: High-Throughput Sequencing of Oligosaccharide Modifications to Proteins....Pages 207-228
Proteomics Databases....Pages 229-257
Quo Vadis....Pages 259-270
Back Matter....Pages 271-274

✦ Subjects

Proteomics; Cell Biology; Human Genetics; Biotechnology; Molecular Medicine; Cancer Research

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