Proteome analysis of round-headed and normal spermatozoa by 2-D fluorescence difference gel electrophoresis and mass spectrometry
โ Scribed by Liao, Ting-Ting; Xiang, Zhen; Zhu, Wen-Bing; Fan, Li-Qing
- Book ID
- 109832395
- Publisher
- Nature Publishing Group
- Year
- 2009
- Tongue
- English
- Weight
- 784 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1008-682X
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Separation and relative quantitation of complex protein mixtures remain two of the most challenging aspects of proteomics. Here an advanced technique called fluorescence difference 2-D gel electrophoresis technology (2D-DIGE) has been applied to a model system study of the Escherichia coli proteome
Careful examination of current literature, particularly over the last 5 years, reveals a wide range of approaches for the relative quantification of protein expression in cells, tissues, and body fluids. In view of such an observation, it is reasonable to ask whether researchers need new methods, or
## Synaptic vesicle proteins under conditions of rest and activation: Analysis by 2-D difference gel electrophoresis Synaptic vesicles are organelles of the nerve terminal that secrete neurotransmitters by fusion with the presynaptic plasma membrane. Vesicle fusion is tightly controlled by depolari