Proteolytic Enzymes: Tools and Targets

✍ Scribed by A. J. Kenny (auth.), Prof. Dr. Erwin E. Sterchi, Prof. Dr. Walter Stöcker (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1999
• Tongue: English
• Leaves: 371
• Series: Springer Lab Manual
• Edition: 1
• Category: Library

✦ Synopsis

Researchers may find themselves confronted with proteases, either because they play an essential role in a particular process they are studying, or because they interfere with that process. In either case they may need to investigate or inhibit the proteolytic activity. Others may wish to use proteolytic enzymes as laboratory tools.
This book has been written with these investigators in mind and includes assay methods using natural and artificial substrates, genetic-based assays, and strategies for the inhibition, purification and crystallization of proteases. In selected chapters the use of proteolytic enzymes to analyze proteins, segregate cells or in peptide synthesis is covered.

✦ Table of Contents

Front Matter....Pages I-IX
Introduction: Nomenclature and Classes of Peptidases....Pages 1-8
Front Matter....Pages 9-9
Chromogenic Peptide Substrates....Pages 11-29
Fluorometric Assays....Pages 30-48
Detection of Proteolytic Enzymes Using Protein Substrates....Pages 49-62
Zymography, Casein Zymography, and Reverse Zymography: Activity Assays for Proteases and their Inhibitors....Pages 63-76
Genetic - Based Assays of Viral Proteases....Pages 77-89
Strategies for Inhibiting Proteases of Unknown Mechanism....Pages 90-106
Front Matter....Pages 107-107
Purification of Proteases....Pages 109-123
Crystallization of Proteinases....Pages 124-147
Basic Kinetic Mechanisms of Proteolytic Enzymes....Pages 148-166
Kinetic Analysis of Protease Inhibition by Synthetic Inhibitors....Pages 167-187
Phosphinic Peptide Libraries for Proteolytic Enzymes....Pages 188-198
Functional Expression of Recombinant Proteases....Pages 199-229
Front Matter....Pages 231-231
Proteases in Peptide Mapping and Sequencing....Pages 233-252
Limited Proteolysis in the Study of Protein Conformation....Pages 253-280
Limited Proteolysis in the Study of Membrane Proteins....Pages 281-297
Segregating Cells - Proteases in Tissue Culture....Pages 298-311
Protease-Catalyzed Peptide Synthesis....Pages 312-328
Back Matter....Pages 329-366

✦ Subjects

Biochemistry, general; Cell Biology