Proteolytic activity of human erythrocyte membrane during ghosts preparation
✍ Scribed by Maria Gaczyńska; Grzegorz Bartosz
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 314 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0020-711X
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A simple procedure for preparing erythrocyte membranes with low basal Ca2+ ATPase activity is described, which is stimulated several-fold by the addition of hemolysate in the incubation mixture. The cells are hemolyzed in hypotonic imidazole buffer and resulting membranes are washed with hypotonic p
The vanadate-sensitive Mg"-dependent ATPase activity of the human erythrocyte ghost is believed to be involved in the shape change events that convert echinocytic ghosts to smoothed forms (biconcave discs and stomatocytes). At physiological salt concentration, pH 7.4,2 m M ATP, 5 m M Mg2' and 1 m M
## Abstract Ghost membranes prepared from human erythrocytes exhibit 2 distinct (Ca+Mg)‐ATPase^1^ activities (Quist and Roufogalis, Arch Biochem Biophys 168:240, 1975). (Ca+Mg)‐ATPase activity dependent on a water soluble protein fraction is selectively lost from ghost membranes during preparation