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Proteoglycans in human burn hypertrophic scar from a patient with Ehlers-Danlos syndrome

✍ Scribed by Hari G. Garg; Eric W. Lippay; Peter J. Neame

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 633 KB
Volume: 223
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(92)80017-u

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✦ Synopsis

Proteoglycans (PGs) from human burn hypertrophic scar of a patient with Ehlers-Danlos syndrome were extracted with 4M guanidinium chloride and purified by DEAE-cellulose chromatography. Differential ethanol precipitation of the PG fraction obtained after ion-exchange chromatography yielded two low mol.-wt. PGs, on rich in glucuronic acid (PGGLCA; Mr 66 kDa) and the other rich in iduronic acid (PGIDOA; Mr 48 kDa). In PGGLCA, 84% of the glycosaminoglycan chains are composed of GlcA----GalNAc(SO4) units, whereas in PGIDOA, the chains contain 95% IdoA----GalNAc(SO4) disaccharide units. Upon treatment with testicular hyaluronidase, the PGs gave different-sized oligosaccharides. Chondroitinase ABC digestion of PGGLCA or PGIDOA gave a single protein core (Mr approximately 20 kDa). The presence of glucosamine and sialic acid in PGGLCA and PGIDOA suggests that both contain N-linked oligosaccharides.

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