✦ LIBER ✦

Proteins from melanosomes of mouse and chick pigment cells

✍ Scribed by Philip Doezema

Publisher: John Wiley and Sons
Year: 1973
Tongue: English
Weight: 659 KB
Volume: 82
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040820108

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✦ Synopsis

Abstract

Purified subcellular fractions containing melanosomes from B‐16 mouse melanoma were treated with 2% sodium dodecyl sulfate or 0.5 M sodium hydroxide to dissolve protein. Quantitative measurements indicate that each melanosome contains 0.065×10^−10^ of protein or about 19% by weight. SDS acrylamide gel electrophoresis of proteins from purified melanosomes resolved six polypeptide bands of major density and about 15 minor bands. These results indicate that the melanosome may be more complex than previous genetic, biochemical or morphological evidence had suggested.

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