The reagent sym-triazine trichloride is used as a bifunctional reagent to generate RNA-protein cross-links within intact ribosomal subunits from E. coli. The reaction takes place in a stepwise manner, involving substitution of one chlorine atom at 12 degrees and pH 8, and substitution of the second
✦ LIBER ✦
Protein−RNA Cross-Linking in the Ribosomes of Yeast under Oxidative Stress
✍ Scribed by Mirzaei, Hamid; Regnier, Fred
- Book ID
- 118233303
- Publisher
- American Chemical Society
- Year
- 2006
- Tongue
- English
- Weight
- 992 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1535-3893
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