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Protein–Protein Binding Affinities in Solution Determined by Electrospray Mass Spectrometry

✍ Scribed by Jiangjiang Liu; Lars Konermann

Book ID
107700624
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
430 KB
Volume
22
Category
Article
ISSN
1044-0305

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## Abstract We describe an approach for the determination of binding constants for protein–ligand complexes with electrospray ionization mass spectrometry, based on the observation of unbound ligands competing for binding to a protein target. For the first time, dissociation constants lower than pi