Protein—nucleic acid interactions in bacteriophage φ29 DNA replication
✍ Scribed by M. Salas; R. Freire; M.S. Soengas; J.A. Esteban; J. Méndez; A. Bravo; M. Serrano; M.A. Blasco; J.M. Lázaro; L. Blanco; C. Gutiérrez; J.M. Hermoso
- Book ID
- 113288119
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 854 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1574-6976
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From +29-infected Bacillus subtilis cells, we have isolated a protein fraction which promotes in vitro replication of +29 DNA. This fraction catalyses both initiation and elongation, indicating that it contains the product of gene 3 (tp: terminal protein) and the product of gene 2 (gp2: probably a D
Cell-free extracts prepared from phi 29 and M2-infected Bacillus subtilis cells catalyse the formation of complexes between terminal protein and [alpha-32P]-dAMP in the presence of [alpha-32P]-dATP, MgCl2, ATP, and phage DNA with terminal protein covalently linked at both the 5'ends. The complex for