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Protein–nucleic acid interactions: from A(rgonaute) to X(PF)

✍ Scribed by Wei Yang; Gregory D Van Duyne

Book ID
113883052
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
70 KB
Volume
16
Category
Article
ISSN
0959-440X

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A rapid mixing-photocrosslinking technique has been developed to investigate the kinetics of protein-nucleic acid interactions. With this technique, binding of nucleic acid to protein is first synchronized by rapid mixing in a stopped-flow apparatus. The intermediates formed at different stages of t