Proteinkristalle und Tubuli-Bündel in Hefezellen III. Identifizierung der Kristalle als Alkoholdehydrogenase (ADH)

✍ Scribed by Dr. W. Künkel

• Book ID: 102909174
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 953 KB
• Volume: 16
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19760160303

✦ Synopsis

Abstract

Inducibility of alcohol dehydrogenase (ADH) crystals in yeast cells depends on the growth phase. They accumulate during exponential growth, a process that is parallelled by accumulation of a special protein (H protein). The following pieces of evidence show that crystals, H protein, and ADH are identical:

The three proteins have the same amino acid composition and subunit molecular weight;

active H protein and ADH exhibit the same electrophoretic mobility, indicating a common molecular weight of about 158000 D;

the native H protein and a crystal fraction stabilized by Zn^++^ and dialyzed against EDTA possess ADH activity.