✦ LIBER ✦

Protein Unfolding and Refolding Under Force: Methodologies for Nanomechanics

✍ Scribed by Bruno Samorì; G. Zuccheri; P. Baschieri

Publisher: John Wiley and Sons
Year: 2004
Tongue: English
Weight: 147 KB
Volume: 6
Category: Article
ISSN: 1439-4235
DOI: 10.1002/cphc.200400343

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✦ Synopsis

Abstract

An increasing number of inter‐ and intramolecular interactions can nowadays be probed using single‐molecule manipulation techniques. Protein unfolding and refolding is the most representative—though complex—of these interactions. Herein, we review the main modes of performing a force unfolding experiment: the velocity clamp and the new force clamp mode. We also compare some of the physical aspects behind the two most frequently used single‐molecule manipulation instrumentations: optical tweezers and atomic force microscopes.

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Studies of protein unfolding and refolding may help us understand the more general problem of protein folding. Recent studies from this laboratory demonstrated that the unfolding and refolding of a large protein, rabbit muscle aldolase (M r 157 kDa), can be studied by combining amide hydrogen exchan