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Protein tryptophan accessibility studied by fluorescence quenching

✍ Scribed by Matías Möller; Ana Denicola

Publisher
The American Society for Biochemistry and Molecular Biology
Year
2002
Tongue
English
Weight
87 KB
Volume
30
Category
Article
ISSN
1470-8175

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✦ Synopsis

Abstract

A laboratory class is described to introduce the biochemistry major students to the basic concepts and various applications of fluorescence spectroscopy. Through simple and inexpensive experiments the students learn how to record excitation and emission spectra and measure intrinsic protein fluorescence and its quenching to elucidate the local tryptophan environment. Free tryptophan, ovalbumin, and bovine serum albumin are used for the experiments. The structural information that can be retrieved from emission spectra, as well as from quenching data, is discussed.

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