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Protein Targeting Protocols || Intracellular Protein Localization by Immunoelectron Microscopy

✍ Scribed by Giezen, Mark


Book ID
121510348
Publisher
Humana Press
Year
2007
Tongue
English
Weight
429 KB
Category
Article
ISBN
1597454664

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Localization of proteins on viral nucleo
✍ Murti, K. G. ;Portner, A. ;Troughton, K. ;Deshpande, K. πŸ“‚ Article πŸ“… 1985 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 871 KB

We have described an indirect immunoelectron microscope method to localize individual proteins on viral nucleocapsids using monoclonal antibodies and colloidal gold-conjugated second antibodies. The procedure provides good binding and retention of antibodies, good resolution( < 24nm), and negligible