Protein synthesis in murine organs during postimplantation development detected by two-dimensional gel electrophoresis

✍ Scribed by Claudia Praxmayer; Karl-Friedrich Murach; Bernhard Baumgartner; Friedrich Aberger; Elisabeth Schlegel; Prof. Karl Illmensee

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 370 KB
• Volume: 13
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.11501301153

✦ Synopsis

Protein synthesis in murine organs during postimplantation development detected by two-dimensional gel electrophoresis

Mouse embryos were isolated from the uterus o n days 10 to 11 ofgestation and incubated in Dulbecco's modified Eagle's medium (DMEM) with ["Slmethionine for 4 h. Subsequently, their hearts and the brains were dissected. The brain was divided into three parts, containing the telencephalon, mesencephalon, and myelencephalon. These tissues were then processed for two-dimensional (2-D) gel electrophoresis. Protein synthesis of the isolated tissues was analyzed for organand cell lineage-specific patterns. We studied proteins with isoelectric points (PI) ranging from 4 to 10 and relative molecular weights (M,) varying from 10000 to 200000 and found several significant quantitative and qualitative differences between the tissues and the developmental stages analyzed. In particular, we were able to distinguish between protein spots that we now attribute putatively to the corresponding embryonic organs. These differences may reflect some of the organ-and cell lineage-specific changes in protein synthesis and gene expression during early mammalian differentiation.