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Protein Synthesis in Giant Liposomes Using the In Vitro Translation System of Thermococcus kodakaraensis

โœ Scribed by Yamaji, K.; Kanai, T.; Nomura, S.-I.M.; Akiyoshi, K.; Negishi, M.; Yong Chen; Atomi, H.; Yoshikawa, K.; Imanaka, T.


Book ID
119820066
Publisher
IEEE
Year
2009
Tongue
English
Weight
876 KB
Volume
8
Category
Article
ISSN
1536-1241

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We investigated the direct constitution of membrane proteins into giant liposomes in cell-free (in vitro) protein synthesis. Giant liposomes were present in a translation reaction cocktail of a wheat germ cell-free protein translation system. Apo cytochrome b 5 (b5) and its fusion proteins were synt