Protein synthesis in eukaryotic organisms: New insights into the function of translation initiation factor EIF-3
✍ Scribed by Ernest M. Hannig
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 587 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0265-9247
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✦ Synopsis
The pathway for initiation of protein synthesis in eukaryotic cells has been defined and refined over the last 25 years using purified components and in vifro reconstituted systems. More recently, powerful genetic analysis in yeast has proved useful in unraveling aspects of translation inherently more difficult to address by strictly biochemical approaches. One area in particular is the functional analysis of multi-subunit protein factors, termed eukaryotic initiation factors (elFs), that play an essential role in translation initiation. elF-3, the most structurally complex of the e l k , has until recently eluded this approach. The identification of the yeast GCD10 gene as the structural gene for the 5 subunit of yeast elF-3(') and the analysis of mutant phenotypes has opened the door to the genetic dissection of the elF-3 protein complex.