High developmental competence of cattle
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Pascal Mermillod; Milan Tomanek; Réjane Marchal; Laurent Meijer
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Article
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2000
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John Wiley and Sons
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English
⚖ 152 KB
Roscovitine, a potent inhibitor of M-phase Promoting Factor (MPF) kinase activity, was used to maintain cattle oocytes at the germinal vesicle stage for a 24-hr culture period. A concentration of 25 M of roscovitine was sufficient to reach the maximum level of meiotic resumption inhibition with 83 Ϯ