𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Protein synthesis and mRNA storage in cattle oocytes maintained under meiotic block by roscovitine inhibition of MPF activity

✍ Scribed by Céline Vigneron; Christine Perreau; Rozenn Dalbiès-Tran; Catherine Joly; Patrice Humblot; Svetlana Uzbekova; Pascal Mermillod


Book ID
111663747
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
195 KB
Volume
69
Category
Article
ISSN
1040-452X

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


High developmental competence of cattle
✍ Pascal Mermillod; Milan Tomanek; Réjane Marchal; Laurent Meijer 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 152 KB

Roscovitine, a potent inhibitor of M-phase Promoting Factor (MPF) kinase activity, was used to maintain cattle oocytes at the germinal vesicle stage for a 24-hr culture period. A concentration of 25 M of roscovitine was sufficient to reach the maximum level of meiotic resumption inhibition with 83 Ϯ