Protein synthesis and germ plasm in cleavage embryos ofXenopus laevis
โ Scribed by Hogarth, K. ;Dixon, K. E.
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 406 KB
- Volume
- 198
- Category
- Article
- ISSN
- 0022-104X
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โฆ Synopsis
Abstract
(^3^H) Leucine was injected into unfertilized eggs, fertilized eggs, and Stage 2โ12 embryos of X. laevis. Incorporation of the leucine into protein by blastomeres containing germ plasm was studied autoradiographically. Eggs, both fertilized and unfertilized, actively synthesized protein, as did embryos from Stage 2 onwards. Probably all blastomers containing germ plasm were labelled. In embryos from Stages 4โ12, the germ plasm itself was also labelled, and this result suggests that the germ plasm is metabolically active during cleavage.
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## Abstract Oocytes of five stages of development were isolated from ovaries of Xenopus laevis and allowed to take up radioactive amino acids. After six hours of labeling the amount of label incorporated into perchloric acid precipitable material from the soluble oocyte fraction and the specific ac