Protein structure and gene organization of mouse lactate dehydrogenase-A isozyme
β Scribed by Steven S.-L. Li; Howard F. Tiano; Kayoko M. Fukasawa; Kiyohito Yagi; Motoyuki Shimizu; Farida S. Sharief; Yasutsugu Nakashima; Yu-ching E. Pan
- Book ID
- 115123051
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 184 KB
- Volume
- 149
- Category
- Article
- ISSN
- 1432-1327
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## Abstract Lactate dehydrogenase (LDH) isozyme 1 (__B__~4~), 5 (__A__~4~), and βXβ (Testis or Sperm type) have been partially purified from mouse tissues. The following studies were carried out on the three isozymes: K~m~ and optimum substrate concentration for pyruvate, Ξ±βoxoβbutyrate, Ξ±βoxoβval
Three homotetrameric lactate dehydrogenase isozymes, LDH-M(A4), LDH-H(B4), and LDH-X(C4), from DBA/2J mice have been purified by affinity chromatography. The amino acid compositions of the subunits A, B, and C, based on a molecular weight of 36,000, have been determined. The compositional relatednes