Protein structure. A practical approach

✍ Scribed by Creighton T.E. (ed.)

• Publisher: IRL Press at Oxford University Press
• Year: 1997
• Tongue: English
• Leaves: 399
• Series: Practical approach series
• Edition: 2ed.
• Category: Library

✦ Synopsis

Crystal Structure Analysis, Third Edition, explains how and why the detailed three-dimensional architecture of molecules can be determined by an analysis of the diffraction patterns obtained when X-rays or neutrons are scattered by the atoms in single crystals. Part 1 covers the nature of the crystalline state, diffraction in general, and diffraction by crystals, and also looks briefly at experimental procedures. Part II examines the problem of converting the experimentally obtained data into a model of the atomic arrangement that scattered these beams. Part III discusses techniques for refining the approximate structure to the degree warranted by the experimental data. It also describes the many types of information that can be obtained by modern crystal structure analysis. The text is enhanced by numerous illustrations, a detailed glossary, and several appendices that cover mathematical details "New and updated throughout, this second edition of Protein Structure presents key electrophoretic, chromatographic, spectrophotometric and immunochemical methods and includes six totally new chapters. Emphasis is placed on the practical aspects of sample preparation and analysis, experimental strategy and design. The companion volume to this book, Protein Function: A Practical Approach (second edition), describes techniques for characterizing protein function." "This new edition of Protein Structure is a major update and extension of the previously highly successful volume and will prove invaluable to all researchers working with proteins, from PhD students to experienced group leaders."--BOOK JACKET.  Read more... Protein molecuar weight determination by sodium docecyl sulfate polyacrylamide gel electrophoresis / Gregory S. Makowski and Melinda L. Ramsby -- Protein analysis by mass spectrometry / Ole NГёrregaard Jensen, Andrej Shevchenko, and Matthias Mann -- Immunological detection of proteins of known sequence / Karl Heinz Scheidmann, Silke Reinartz, and Harold Schlebusch -- Identification of common post-translational modifications / Rahha G. Krishna and Finn Wold -- Peptide mapping / Nick A. Morrice and Elizabeth A. Carrey -- Counting integral numbers of residues by chemical modification / Michelle Hollecker -- Disulfide bonds between cycteine residues / William R. Gray -- Analysis of protein conformation by gel electrophoresis / David P. Goldenberg -- Hydrodynamic properties of proteins / Stephen E. Harding -- How to determine the molar absorbance coefficient of a protein / C. Nick Pace and Franz X. Schmid -- Optical spectroscopy to characterize protein conformation and conformational changes / Franz X. Schmid -- Measuring the conformational stability of a protein / C. Nick Pace and J. Martin Scholtz -- Immunochemical analysis of protein conformation / Bertrand Friiguet, Lisa Djavadi-Ohaniance, and Michel E. Goldberg -- Stabilization of protein structure by solvents / Serge N. Timasheff and Tsutomu Arakawa