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Protein splicing: Excision of intervening sequences at the protein level

โœ Scribed by Antony A. Cooper; To M. H. Stevens

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
984 KB
Volume
15
Category
Article
ISSN
0265-9247

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โœฆ Synopsis

Protein splicing is an extraordinary post-translational reaction that removes an intact central "spacer" domain (Sp) from precursor proteins (N-Sp-C) while splicing together the N-and C-domains of the precursor, via a peptide bond, to produce a new protein (N-C). All of the available data on protein splicing fit a model in which these intervening sequences excise at the protein level via a self-splicing mechanism. Several proteins have recently been discovered that undergo protein splicing, and in two such cases, the excised spacer protein is an endonuclease. Such endonucleases are capable of conferring genetic mobility upon the intervening sequences that encodes them. These intervening sequences define a new family of mobile genetic elements that are translated yet remain phenotypically silent by excising at the protein rather than the RNA level.

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