Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (
Protein Sequencing Protocols Volume 64 || The Dansyl Method for Identifying N-Terminal Amino Acids
โ Scribed by Smith, Bryan John
- Book ID
- 121528663
- Publisher
- Humana Press
- Year
- 1996
- Weight
- 955 KB
- Edition
- 1
- Category
- Article
- ISBN-13
- 9780896033535
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Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (
A highly sensitive new method for identifying the carboxy terminus of a protein was developed. The carboxyl terminal amino acid was racemized by reaction with acetic anhydride. The resulting modified protein was subjected to acid hydrolysis. The hydrolysate was derivatized with (+)-1-(9-fluorenyl)et