Protein Sequencing Protocols
β Scribed by Bryan John Smith, Paul Tempst (auth.), Bryan John Smith (eds.)
- Publisher
- Humana Press
- Year
- 2003
- Tongue
- English
- Leaves
- 488
- Series
- Methods in Molecular Biologyβ’ 211
- Edition
- 2
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
Bryan John Smith updates his much-acclaimed first edition of Protein Sequencing Protocols with new and refreshed techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, but also offers a guide to the analysis of protein sequence information via the powerful new tools of bioinformatics. Each readily reproducible protocol contains detailed step-by-step instructions to ensure successful experimental results, along with notes on troubleshooting and how to avoid pitfalls. Valuable appendices summarize changes in mass arising from protein modification, as well as consensus sequences for protein modification.
Comprehensive and up-to-date, Protein Sequencing Protocols, Second Edition, provides both novice and expert investigators alike a ready source of easy-to-follow protocols that simplify choosing the most appropriate method for protein sequence determination and thus help assure robust results.
β¦ Table of Contents
Front Matter....Pages i-xii
Back Matter....Pages 1-18
....Pages 19-30
β¦ Subjects
Biochemistry, general
π SIMILAR VOLUMES
Bryan Smith draws together in Protein Sequencing Protocols a timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on masters of the procedures. These well-tested methods convey valuable lessons and insights into using
Bryan John Smith updates his much-acclaimed first edition with new and updated techniques for determining the sequence of proteins and peptides. This edition includes not only novel approaches to the validation of quality assurance methods, reflecting the current importance of biopharmaceuticals, bu
A timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on, masters of the procedures. Conveys valuable lessons and insights into using automated sequencers and mass spectrometers, preparing proteins and peptides on a m
<span>Determination of the protein sequence is as important today as it was a half century ago, even though the techniques and purposes have changed over time. Mass spectrometry has continued its recent rapid development to find notable application in the characterization of small amounts of protein