Protein sensors based on the potentiometric photoresponse of polymer membranes doped with photochromic spiropyran

✍ Scribed by Jun-ichi Anzai; Kenji Sakamura; Yasushi Hasebe; Tetsuo Osa

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 416 KB
• Volume: 281
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85013-a

✦ Synopsis

The potentiometric photoresponse of plasticized polymer [carboxylated poly(vinyl chloride)] membranes doped with a spirobenzopyran derivative was modulated significantly by specific adsorption of proteins on the membrane surface. The magnitude of the photoinduced potential of the membranes was decreased in the presence of l-15 pg ml-' of anti-DNP or 0.1-1.5 pg ml-' of avidin in solution. The possible use of the membranes as protein sensor materials is discussed in terms of response time, selectivity and re-usability.