Secretion-dependent proteolysis of heter
✍
Michael Schmidt; Elisenda Viaplana; Frank Hoffmann; Sabine Marten; Antonio Villa
📂
Article
📅
1999
🏛
John Wiley and Sons
🌐
English
⚖ 234 KB
👁 1 views
The synthesis of a proteolytically unstable protein, originally designed for periplasmic export in recombinant Escherichia coli BL21(DE3), a strain naturally deficient for the ATP-dependent protease Lon (or La) and the outer membrane protease OmpT, is associated with a severe growth inhibition. This