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Protein reducing agents dithiothreitol and tris(2-carboxyethyl)phosphine anodic oxidation

✍ Scribed by Santarino, Inês Barreira; Oliveira, Severino Carlos B.; Oliveira-Brett, Ana Maria


Book ID
123505592
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
280 KB
Volume
23
Category
Article
ISSN
1388-2481

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The newly introduced sulfhydryl reductant tris(2-carboxyethyl)phosphine (TCEP) is a potentially attractive alternative to commonly used dithiothreitol (DTT). We compare properties of DTT and TCEP important in protein biochemistry, using the motor enzyme myosin as an example protein. The reductants e